Composite

Part:BBa_K3396017:Design

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)


Replaceable1-Replaceable2-P2A-Truncated Trim21-Replaceable3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1106
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1063
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 840
    Illegal BsaI site found at 1789
    Illegal BsaI.rc site found at 786


Design Notes

In order to simplify the cloning of PREDATOR Pro system, we herein introduced three replaceable regions that can be replaced with respective coding sequences with Golden Gate assembly.


Source

Truncated Trim21 was originally expressed in human.

References